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KMID : 1059520080520060608
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Journal of the Korean Chemical Society
2008 Volume.52 No. 6 p.608 ~ p.613
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Using the Extended Solvation theory for Thermodynamic Study on the interaction of Magnesium and Cobalt ions with human growth hormone
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Behbehani G. Rezaei
Saboury A. A.
Divsalar A.
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Abstract
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A thermodynamic study on the interaction between magnesium and cobalt ions (M2+), and human growth hormone, hGH, was studied at 27 °C in NaCl solution (50 mM) using the isothermal titration calorimetry. Isothermal titration calorimetry was applied to obtain the binding isotherm for hGH+M2+. The results obtained indicate that there is a set of three identical and noninteracting binding sites for Cobalt ions and a set of two for magnesium. The extended solvation model was used to reproduce the enthalpies of M2++hGH interactions over the whole metal ions concentrations. The solvation parameters recovered from the solvation model were attributed to the structural change of hGH due to the metal ion interaction. The extended solvation model was applied to elucidate the effect of Mg2+ and Co2+ binding on the protein stability.
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KEYWORD
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Isothermal Titration Calorimetry, Human Growth Hormone (hGH), Solvation Model
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